tumorigenic human mammary epithelial cell line mcf10a (ATCC)

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ATCC tumorigenic human mammary epithelial cell line mcf10a

Molecular changes associated with LFG‐specific siRNA treatment in MDA‐MB‐231 and <t>MCF10A</t> cells. (A) LFG mRNA expression measured by qRT‐PCR after 24 and 48 h. Expression was normalized to ACTB and calculated using the 2 −ΔΔCt method with MDA‐MB‐231 control cells as the calibrator. Data represent mean ± SD from three independent experiments. * p < 0.05 versus the corresponding control (Student's t ‐test). (B) Representative fluorescence images showing intracellular Ca 2+ ‐associated fluorescence in MDA‐MB‐231 cells following LFG‐specific siRNA transfection. Images shown are representative of three independent biological experiments ( n = 3). Cells were imaged using identical microscope settings, exposure time, gain, and illumination intensity for all experimental groups. Green fluorescence represents intracellular calcium‐associated fluorescence. Scale bar = 200 μm. (C) Relative expression of selected ER stress‐ and apoptosis‐related genes in MDA‐MB‐231 and MCF10A cells 48 h after LFG‐specific siRNA transfection, determined using the RT 2 Profiler PCR Array. Gene expression was normalized to ACTB (β‐actin) and calculated using the 2 −ΔΔCt method with the corresponding non‐targeting siRNA‐transfected cells as the calibrator. Genes with a fold change ≥ 2 or ≤ −2 were considered differentially expressed. Data are presented as mean ± SD from three independent biological experiments ( n = 3). Statistical comparisons were performed using an unpaired two‐tailed Student's t ‐test, with p < 0.05 considered statistically significant. Error bars represent standard deviation (SD). The asterisk indicates statistical significance compared with the control group ( p < 0.05)

Tumorigenic Human Mammary Epithelial Cell Line Mcf10a, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8191 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tumorigenic human mammary epithelial cell line mcf10a - by Bioz Stars, 2026-10

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Images

1) Product Images from "Regulation of ER Stress‐Associated Gene Expression by Lifeguard in Human Breast Cancer Cells"

Article Title: Regulation of ER Stress‐Associated Gene Expression by Lifeguard in Human Breast Cancer Cells

Journal: Cancer Reports

doi: 10.1002/cnr2.70687

Molecular changes associated with LFG‐specific siRNA treatment in MDA‐MB‐231 and MCF10A cells. (A) LFG mRNA expression measured by qRT‐PCR after 24 and 48 h. Expression was normalized to ACTB and calculated using the 2 −ΔΔCt method with MDA‐MB‐231 control cells as the calibrator. Data represent mean ± SD from three independent experiments. * p < 0.05 versus the corresponding control (Student's t ‐test). (B) Representative fluorescence images showing intracellular Ca 2+ ‐associated fluorescence in MDA‐MB‐231 cells following LFG‐specific siRNA transfection. Images shown are representative of three independent biological experiments ( n = 3). Cells were imaged using identical microscope settings, exposure time, gain, and illumination intensity for all experimental groups. Green fluorescence represents intracellular calcium‐associated fluorescence. Scale bar = 200 μm. (C) Relative expression of selected ER stress‐ and apoptosis‐related genes in MDA‐MB‐231 and MCF10A cells 48 h after LFG‐specific siRNA transfection, determined using the RT 2 Profiler PCR Array. Gene expression was normalized to ACTB (β‐actin) and calculated using the 2 −ΔΔCt method with the corresponding non‐targeting siRNA‐transfected cells as the calibrator. Genes with a fold change ≥ 2 or ≤ −2 were considered differentially expressed. Data are presented as mean ± SD from three independent biological experiments ( n = 3). Statistical comparisons were performed using an unpaired two‐tailed Student's t ‐test, with p < 0.05 considered statistically significant. Error bars represent standard deviation (SD). The asterisk indicates statistical significance compared with the control group ( p < 0.05)

Figure Legend Snippet: Molecular changes associated with LFG‐specific siRNA treatment in MDA‐MB‐231 and MCF10A cells. (A) LFG mRNA expression measured by qRT‐PCR after 24 and 48 h. Expression was normalized to ACTB and calculated using the 2 −ΔΔCt method with MDA‐MB‐231 control cells as the calibrator. Data represent mean ± SD from three independent experiments. * p < 0.05 versus the corresponding control (Student's t ‐test). (B) Representative fluorescence images showing intracellular Ca 2+ ‐associated fluorescence in MDA‐MB‐231 cells following LFG‐specific siRNA transfection. Images shown are representative of three independent biological experiments ( n = 3). Cells were imaged using identical microscope settings, exposure time, gain, and illumination intensity for all experimental groups. Green fluorescence represents intracellular calcium‐associated fluorescence. Scale bar = 200 μm. (C) Relative expression of selected ER stress‐ and apoptosis‐related genes in MDA‐MB‐231 and MCF10A cells 48 h after LFG‐specific siRNA transfection, determined using the RT 2 Profiler PCR Array. Gene expression was normalized to ACTB (β‐actin) and calculated using the 2 −ΔΔCt method with the corresponding non‐targeting siRNA‐transfected cells as the calibrator. Genes with a fold change ≥ 2 or ≤ −2 were considered differentially expressed. Data are presented as mean ± SD from three independent biological experiments ( n = 3). Statistical comparisons were performed using an unpaired two‐tailed Student's t ‐test, with p < 0.05 considered statistically significant. Error bars represent standard deviation (SD). The asterisk indicates statistical significance compared with the control group ( p < 0.05)

Techniques Used: Expressing, Quantitative RT-PCR, Control, Fluorescence, Transfection, Microscopy, Gene Expression, Two Tailed Test, Standard Deviation

Cell Culture:

Article Title: C-terminal HSP90 inhibitor NCT-58 impairs the cancer stem-like phenotype and enhances chemotherapy efficacy in TNBC
Article Snippet: The TNBC cell lines MDA-MB-231 (PerkinElmer, Inc.), Hs578T (American Type Culture Collection; ATCC), BT549 and 4T1 (Japanese Collection of Research Bioresources Cell Bank), and the cell line 293 (Korean Cell line Bank) were maintained in MEM or RPMI-1640 supplemented with 10% FBS and 100 U/ml penicillin-streptomycin (Gibco; Thermo Fisher Scientific, Inc.). .. The normal human mammary epithelial cell line MCF10A (ATCC) was cultured in Mammary Epithelial Cell Growth Basal Medium, including hEGF (20 ng/ml), insulin (10 μg/ml), hydrocortisone (0.5 μg/ml) and bovine pituitary extract (50 μg/ml) (SingleQuots TM Kit; Lonza Group, Ltd.) containing streptomycin-penicillin (100 U/ml). ..

Article Title: Integrative characterization of MYC RNA-binding function.
Article Snippet: Mouse embryonic fibroblast cell line NIH/3T3 (CRL-1658) was obtained from the American Type Culture Collection (ATCC) and cultured in Dulbecco’s Modified Eagle Medium (DMEM, HyClone, SH30243.01) supplemented with 10% fetal bovine serum (FBS, Gibco, 10438-026), 1% MEM non-essential amino acids (Gibco, 11140-050), 10 mM HEPES (Gibco, 15630-080), and 1% penicillin/streptomycin (Corning, 30-002-CI). .. Human mammary epithelial cell line MCF10A (CRL-10317) was obtained from ATCC and cultured in 1:1 DMEM/F-12 (Gibco, 11320-033) supplemented with 5% horse serum (Gibco, 16050-122), 20 ng/mL human EGF (Peprotech, AF-100-15), 0.5 μg/mL hydrocortisone (Sigma, H0888), 100 ng/mL cholera toxin (Sigma, C8052), 10 μg/mL insulin (Sigma, I1882), and 1% penicillin/streptomycin. .. Mouse embryonic fibroblast cell line NIH/3T3 (CRL-1658) was obtained from the American Type Culture Collection (ATCC) and cultured in Dulbecco’s Modified Eagle Medium (DMEM, HyClone, SH30243.01) supplemented with 10% fetal bovine serum (FBS, Gibco, 10438-026), 1% MEM non-essential amino acids (Gibco, 11140-050), 10 mM HEPES (Gibco, 15630-080), and 1% penicillin/streptomycin (Corning, 30-002-CI).

Article Title: Breast Cancer Cells Exhibit Mesenchymal-Epithelial Plasticity Following Dynamic Modulation of Matrix Stiffness.
Article Snippet: .. Cell Culture: The human breast cancer cell lines MDA-MB-231 (HTB26) and MCF7 (HTB-22) and human mammary epithelial cell line MCF10A (CRL-10317) were purchased from the American Type Culture Collection (ATCC). .. The cells were grown according to the recommendations of ATCC.

Article Title: Integrative characterization of MYC RNA-binding function
Article Snippet: Mouse embryonic fibroblast cell line NIH/3T3 (CRL-1658) was obtained from the American Type Culture Collection (ATCC) and cultured in Dulbecco’s Modified Eagle Medium (DMEM, HyClone, SH30243.01) supplemented with 10% fetal bovine serum (FBS, Gibco, 10438-026), 1% MEM non-essential amino acids (Gibco, 11140-050), 10 mM HEPES (Gibco, 15630-080), and 1% penicillin/streptomycin (Corning, 30-002-CI). .. Human mammary epithelial cell line MCF10A (CRL-10317) was obtained from ATCC and cultured in 1:1 DMEM/F-12 (Gibco, 11320-033) supplemented with 5% horse serum (Gibco, 16050-122), 20 ng/mL human EGF (Peprotech, AF-100-15), 0.5 μg/mL hydrocortisone (Sigma, H0888), 100 ng/mL cholera toxin (Sigma, C8052), 10 μg/mL insulin (Sigma, I1882), and 1% penicillin/streptomycin. .. Human cell lines, including osteosarcoma cell line U2OS (HTB-96), leukemia cell line K562 (CCL-243), colorectal carcinoma cell line HCT116 (CCL-247), hepatocellular carcinoma cell line HepG2 (HB-8065), breast adenocarcinoma cell line MCF-7 (HTB-22), and human embryonic kidney (HEK) cell line 293T (CRL-3216), were obtained from ATCC and cultured according to ATCC’s guidelines.

Article Title: Therapeutic targeting of circTNK2 with nanoparticles restores tamoxifen sensitivity and enhances NK cell-mediated immunity in ER-positive breast cancer.
Article Snippet: Please cite this article as: R. Wu, S. Yu, A. Bi, Y. Li, D. Tiek, K. Yu, H. Xiong, Q. Shi, Z. Mo, X. Yu, X.. Song, F. Yin, Y. Wang, W. Yi, M. Liu, P. Li, B. Hu, A.. Le, S.-Y.

Multiple Displacement Amplification:

Article Title: MYC-bound enhancer RNAs in cis regulate gene transcription and tumorigenesis
Article Snippet: .. Human breast cancer cell lines, including BT-20, BT474, HCC1937, Hs578T, MCF-7, MDA-MB-231 (MB231), MDA-MB-361 (MB361), MDA-MB-468 (MB468), T-47D, and ZR-75-1, and human mammary epithelial cell line MCF10A, human lung cancer cell line A549, and human embryonic kidney (HEK) 293T cell line were directly obtained from the American Type Culture Collection (ATCC). .. BT-20 was maintained in Eagle’s minimum essential medium (EMEM; Corning, 10-009-CV) supplemented with 10% fetal bovine serum (FBS; Gibco, A5256801) and 1% penicillin/streptomycin (PS; Corning, 30-002-CI).

Article Title: LncRNA TYMSOS facilitates breast cancer metastasis and immune escape through downregulating ULBP3
Article Snippet: .. Human mammary epithelial cell line MCF10A and human breast cancer cell lines MCF-7, Hs578T, T47D, MDA-MB-231, MDA-MB-468 cells and human NK cell line NK92 cells were from ATCC (Manassas, VA, USA). .. MCF10A cells were cultured in F12/DMEM (Gibco, Grand Island, NY, USA).

other:

Article Title: Integrative characterization of MYC RNA-binding function.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pLIX-Flag-MYC7A doxycycline-inducible This study N/A pET28b Novagen Cat# 69865-3 pET28b-His-MAX This study N/A pET28b-His-MYCWT This study N/A pET28b-His-MYCKRR3A This study N/A pET28b-His-MYCRQRR4A This study N/A pET28b-His-MYC7A This study N/A pET28b-His-MYCΔBR This study N/A pEGFP-C1 Clontech Cat# 6084-1 lenti dCAS9-VP64_Blast Konermann et al.56 Addgene# 61425 lenti dCas9_Blast This study N/A pLKO.1-puro U6 sgRNA BfuAI Kearns et al.57 Addgene# 50920 pLKO.1-puro U6 gRNA This study N/A pLKO.1-puro U6 gRNA-GFP This study N/A pLKO.1-puro U6 gRNA-eGREB1 This study N/A pLKO.1-puro U6 gRNA-eGREB1-probe This study N/A pLKO.1-puro U6 gRNA-eGREB1-probe G7C This study N/A pLKO.1-puro U6 gRNA-eGREB1-probe G8C This study N/A Software and algorithms Prism Version 9.3.1 GraphPad Software https://www.graphpad.com ImageJ NIH https://imagej.nih.gov/ij/ Bowtie2 v2.5.1 Langmead et al.58 http://bowtie-bio.sourceforge.net/ bowtie2/index.shtml MACS2 Zhang et al.59 https://github.com/macs3-project/MACS CTK Shah et al.60 https://zhanglab.c2b2.columbia.edu/ index.php/CTK_Documentation BWA v0.7.17 Li and Durbin.61 http://bio-bwa.sourceforge.net/ HOMER v4.11.1 Heinz et al.62 http://homer.ucsd.edu/homer/ Gseapy Subramanian et al.63 https://gseapy.readthedocs.io/en/latest/ Samtools v1.13 Li et al.64 https://github.com/samtools/ Bedtools Quinlan et al.65 https://bedtools.readthedocs.io/en/latest/ deeptools v3.5.4.post1 Ramı́rez et al.66 https://deeptools.readthedocs.io/en/develop/ Enrichr Chen et al.67 https://gseapy.readthedocs.io/en/latest/ Cutadapt v4.4 Martin68 https://cutadapt.readthedocs.org/ Python N/A https://www.python.org PEKA Kuret et al.30 https://github.com/ulelab/peka MEME v5.5.4 Bailey et al.31 https://meme-suite.org/meme/ STAR v2.7.11a Dobin et al.69 https://github.com/alexdobin/STAR Cufflinks v2.2.1 Trapnell et al.70 https://cole-trapnell-lab.github.io/cufflinks/ e4 Cell Genomics 5, 100878, July 9, 2025 Article ll OPEN ACCESS (CCL-243), colorectal carcinoma cell line HCT116 (CCL-247), hepatocellular carcinoma cell line HepG2 (HB-8065), breast adenocarcinoma cell line MCF-7 (HTB-22), and human embryonic kidney (HEK) cell line 293T (CRL-3216), were obtained from ATCC and cultured according to ATCC’s guidelines.